<html><head><style type="text/css"><!-- DIV {margin:0px;} --></style></head><body><div style="font-family:times new roman,new york,times,serif;font-size:12pt">Hi All-<br><br>I have a set of images of a c-elegans neuron, from which I want to characterize the movement of the axon. The images are florescence microscopy images and only the axon lights up in these images. I am able to segment out the axon, but am not able to find an appropriate filter to fit a curve to the binary image such that the curve fits the axon. I have seen the itkContourExtractor2DImageFilter, but I am unable to visualize the Paths that this filter generates. So I have two questions for the list<br><br>(1) The ContourExtractor2DImageFilter tries to extract closed contours from the image and makes these available as a PolyLineParametricPath<2>. Is there a way to render the curves extracted using VTK? <br><br>Also I suspect that it is taking the dots that represent
the axon and generating a set of closed contours that run around the dots as opposed to fitting all the dots into a single path (from what I understand of marching squares). This would make it unsuitable for my purpose. Any comments to the corroborating either this observation or its contrary are appreciated.<br><br>(2) Is there a filter to which I can feed as input either the binary image that represents the axon, or a point set derived from the binary image,from which a Bspline can be derived? In the ideal case I would like the output to be in the form of a closed form charecterization (BSpline etc) that I can sample at various points and create a TubeSpatialObject to represent the axon.<br><br>Thanks,<br>--Prashanth Ravindran<br></div></body></html>