[Insight-users] The Insight Journal-New Submission
Insight Journal
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Wed Mar 11 11:07:54 EDT 2009
Hello,
A new submission has been added to the Insight Journal.
Title: Microscopy Image Analysis: Blob Segmentation using Geodesic Active Contours
Author(s):Mosaliganti k., gelas a., gouaillard a., Megason S.
Abstract:
An Insight Toolkit (ITK) processing framework for blob segmentation with applications to microscopy
images is presented in this paper. Our algorithm is a reï¬nement of the work of Mosaliganti et al. [3] for
splitting cell clusters. The basic idea is to incorporate as many cues as possible into developing a suitable
level-set speed function so that an evolving contour exactly segments a cell/nuclei blob. We use image
gradients, distance maps, multiple channel information and a shape model to drive the evolution. The
framework consists of a linear pipeline of 6 new ITK ï¬lters which are applied in succession to generate
the segmentation. The ï¬lters extract the cell foreground, construct the speed image, ï¬nd seed points for
evolution and collect cell statistics from the segmentation. We include 2D/3D example code, parameter
settings and show the results generated on confocal images of the zebraï¬sh embryo.
Download and Review the paper at: http://www.insight-journal.org/browse/publication/321
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