[Insight-users] The Insight Journal-New Submission

Insight Journal webmaster at insightsoftwareconsortium.org
Wed Mar 11 11:07:54 EDT 2009


Hello,
A new submission has been added to the Insight Journal.

Title: Microscopy Image Analysis: Blob Segmentation using Geodesic Active Contours
Author(s):Mosaliganti k., gelas a., gouaillard a., Megason S.
Abstract: 
An Insight Toolkit (ITK) processing framework for blob segmentation with applications to microscopy 
images is presented in this paper. Our algorithm is a refinement of the work of Mosaliganti et al. [3] for 
splitting cell clusters. The basic idea is to incorporate as many cues as possible into developing a suitable 
level-set speed function so that an evolving contour exactly segments a cell/nuclei blob. We use image 
gradients, distance maps, multiple channel information and a shape model to drive the evolution. The 
framework consists of a linear pipeline of 6 new ITK filters which are applied in succession to generate 
the segmentation. The filters extract the cell foreground, construct the speed image, find seed points for 
evolution and collect cell statistics from the segmentation. We include 2D/3D example code, parameter 
settings and show the results generated on confocal images of the zebrafish embryo.

Download and Review the paper at: http://www.insight-journal.org/browse/publication/321

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