[Insight-users] bioformats and ITK
Kishore Mosaliganti
kishoreraom at gmail.com
Fri Oct 12 08:53:58 EDT 2012
Hi all,
I am trying to use bioformats and itk. I downloaded and compiled
bf-itk-pipe using cmake by linking against ITK 3.2:
https://github.com/openmicroscopy/bioformats/tree/develop/components/native/bf-itk-pipe
I tested out itkBFImageInfo and ./itkRGBBioFormatsImageIOTest on a few
simple LSM images. It works great and writes out all the associated
metadata and pixel data for the first timepoint.
My LSM microscopy image is 5D. It is X-Y-Z-Channel-Time. I am interested in
extracting individual timepoints from the LSM file for processing. The
filter seems to be using itkStreamingImageFilter. I set the NumberOfStreams
to 10 but that still seems to still write out only the first image.
How do I set the output requestion region for an individual timepoint?
Thank you,
Kishore
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