Examples/Filtering/DiffusionTensor3DReconstructionImageFilter.cxx

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//
// This example shows how to use the
// DiffusionTensor3DReconstructionImageFilter to reconstruct an image of
// tensors from Diffusion weighted images. See the documentation of
// DiffusionTensor3DReconstructionImageFilter,
// TensorRelativeAnisotropyImageFilter, TensorFractionalAnisotropyImageFilter
// first.
//
// The example takes diffusion weighted images in the Nrrd format, writes out
// the gradient and reference images for the users reference and computes and
// writes out the Fractional Anisotropy and Relative Anisotropy images.
//
// The easiest way to get started is to try out the filter on a sample
// dataset.
//
// Acquiring sample datasets:
//  1. Get the DWI datasets from
//        ftp://public.kitware.com/pub/namic/DTI/Data/dwi.nhdr
//        ftp://public.kitware.com/pub/namic/DTI/Data/dwi.img.gz (gunzip this)
//     These datasets contain a reference T1 image and 30 diffusion weighted
//     images. See the nrrd header for details such as B value etc.
//
//  2. Run the example with the following args
//       dwi.nhdr 80 Tensors.mhd FractionalAnisotropy.mhd
//       RelativeAnisotropy.mhd 1
//
//  3. You should find 30 gradient images, 1 reference image, the FA and RA
//  images
//     in your working directory, which you can fire up in your favourite
//     volume browser.
//
// This work is part of the National Alliance for Medical Image
// Computing (NAMIC), funded by the National Institutes of Health
// through the NIH Roadmap for Medical Research, Grant U54 EB005149.
//
// Additional documentation: For details on the Nrrd format for DTI, see
// https://wiki.na-mic.org/Wiki/index.php/NAMIC_Wiki:DTI:Nrrd_format
//
 
 
#include "itkDiffusionTensor3DReconstructionImageFilter.h"
#include "itkTensorFractionalAnisotropyImageFilter.h"
#include "itkTensorRelativeAnisotropyImageFilter.h"
#include "itkNrrdImageIO.h"
#include "itkImageSeriesReader.h"
#include "itkImageFileWriter.h"
#include "itkImageRegionIterator.h"
#include <iostream>
 
int
main(int argc, char * argv[])
{
  if (argc < 3)
  {
    std::cerr << "Usage: " << argv[0]
              << " NrrdFileName(.nhdr) threshold(on B0)"
              << " FAImageFileName RelativeAnisotropyFileName "
              << "[ExtractGradientAndReferenceImage from the NRRD file and "
              << "write them as images]" << std::endl;
    std::cerr << "\tExample args: xt_dwi.nhdr 80 FA.mhd 1" << std::endl;
    return EXIT_FAILURE;
  }
 
  constexpr unsigned int Dimension = 3;
  unsigned int           numberOfImages = 0;
  unsigned int           numberOfGradientImages = 0;
  bool                   readb0 = false;
  double                 b0 = 0;
 
  using PixelType = unsigned short;
  using ImageType = itk::VectorImage<unsigned short, 3>;
 
  itk::ImageFileReader<ImageType>::Pointer reader =
    itk::ImageFileReader<ImageType>::New();
 
  ImageType::Pointer img;
 
  // Set the properties for NrrdReader
  reader->SetFileName(argv[1]);
 
  // Read in the nrrd data. The file contains the reference image and the
  // gradient images.
  try
  {
    reader->Update();
    img = reader->GetOutput();
  }
  catch (const itk::ExceptionObject & ex)
  {
    std::cout << ex << std::endl;
    return EXIT_FAILURE;
  }
 
  // Here we instantiate the DiffusionTensor3DReconstructionImageFilter class.
  // The class is templated over the pixel types of the reference, gradient
  // and the to be created tensor pixel's precision. (We use double here). It
  // takes as input the Reference (B0 image acquired in the absence of
  // diffusion sensitizing gradients), 'n' Gradient images and their
  // directions and produces as output an image of tensors with pixel-type
  // DiffusionTensor3D.
  //
  using TensorReconstructionImageFilterType = itk::
    DiffusionTensor3DReconstructionImageFilter<PixelType, PixelType, double>;
 
 
  // -------------------------------------------------------------------------
  // Parse the Nrrd headers to get the B value and the gradient directions
  // used for diffusion weighting.
  //
  // The Nrrd headers should look like :
  // The tags specify the B value and the gradient directions. If gradient
  // directions are (0,0,0), it indicates that it is a reference image.
  //
  // DWMRI_b-value:=800
  // DWMRI_gradient_0000:= 0 0 0
  // DWMRI_gradient_0001:=-1.000000       0.000000        0.000000
  // DWMRI_gradient_0002:=-0.166000       0.986000        0.000000
  // DWMRI_gradient_0003:=0.110000        0.664000        0.740000
  // ...
  //
  itk::MetaDataDictionary  imgMetaDictionary = img->GetMetaDataDictionary();
  std::vector<std::string> imgMetaKeys = imgMetaDictionary.GetKeys();
  std::vector<std::string>::const_iterator itKey = imgMetaKeys.begin();
  std::string                              metaString;
 
  TensorReconstructionImageFilterType::GradientDirectionType vect3d;
  TensorReconstructionImageFilterType::GradientDirectionContainerType::Pointer
    DiffusionVectors = TensorReconstructionImageFilterType::
      GradientDirectionContainerType::New();
 
 
  for (; itKey != imgMetaKeys.end(); ++itKey)
  {
    double x, y, z;
 
    itk::ExposeMetaData<std::string>(imgMetaDictionary, *itKey, metaString);
    if (itKey->find("DWMRI_gradient") != std::string::npos)
    {
      std::cout << *itKey << " ---> " << metaString << std::endl;
      sscanf(metaString.c_str(), "%lf %lf %lf\n", &x, &y, &z);
      vect3d[0] = x;
      vect3d[1] = y;
      vect3d[2] = z;
      DiffusionVectors->InsertElement(numberOfImages, vect3d);
      ++numberOfImages;
      // If the direction is 0.0, this is a reference image
      if (vect3d[0] == 0.0 && vect3d[1] == 0.0 && vect3d[2] == 0.0)
      {
        continue;
      }
      ++numberOfGradientImages;
    }
    else if (itKey->find("DWMRI_b-value") != std::string::npos)
    {
      std::cout << *itKey << " ---> " << metaString << std::endl;
      readb0 = true;
      b0 = std::stod(metaString.c_str());
    }
  }
  std::cout << "Number of gradient images: " << numberOfGradientImages
            << " and Number of reference images: "
            << numberOfImages - numberOfGradientImages << std::endl;
  if (!readb0)
  {
    std::cerr << "BValue not specified in header file" << std::endl;
    return EXIT_FAILURE;
  }
 
 
  // -------------------------------------------------------------------------
  // Extract the Reference and gradient images from the NRRD file
  // as separate images.
  //
  // This is not really necessary, the filter is capable of gobbling the
  // entire VectorImage (which contains the reference and the gradient image)
  // and chew on it to generate the TensorImage. This is a more intuitive
  // formalism since this is what is usually obtained from the Nrrd DWI
  // format.
  //
  // Nevertheless, we go through the "unnecessary pain" of extracting the
  // gradient and reference images in separate images and writing them out to
  // files, so they can be fired up in your favourite volume viewer.
  //
  using ReferenceImageType = itk::Image<PixelType, Dimension>;
  using GradientImageType = ReferenceImageType;
 
  // A container of smart pointers to images. This container will hold
  // the 'numberOfGradientImages' gradient images and the reference image.
  //
  std::vector<GradientImageType::Pointer> imageContainer;
 
  // iterator to iterate over the DWI Vector image just read in.
  using DWIIteratorType = itk::ImageRegionConstIterator<ImageType>;
  DWIIteratorType dwiit(img, img->GetBufferedRegion());
  using IteratorType = itk::ImageRegionIterator<GradientImageType>;
 
  // In this for loop, we will extract the 'n' gradient images + 1 reference
  // image from the DWI Vector image.
  //
  for (unsigned int i = 0; i < numberOfImages; ++i)
  {
    auto image = GradientImageType::New();
    image->CopyInformation(img);
    image->SetBufferedRegion(img->GetBufferedRegion());
    image->SetRequestedRegion(img->GetRequestedRegion());
    image->Allocate();
 
    IteratorType it(image, image->GetBufferedRegion());
    dwiit.GoToBegin();
    it.GoToBegin();
 
    while (!it.IsAtEnd())
    {
      it.Set(dwiit.Get()[i]);
      ++it;
      ++dwiit;
    }
    imageContainer.push_back(image);
  }
 
  // If we need to write out the reference and gradient images to a file..
  // Easier viewing them with a viewer than if they were in a single NRRD
  // file
  if ((argc > 4) && (std::stoi(argv[6])))
  {
    unsigned int referenceImageIndex = 0;
    using GradientWriterType = itk::ImageFileWriter<GradientImageType>;
    for (unsigned int i = 0; i < numberOfImages; ++i)
    {
      auto gradientWriter = GradientWriterType::New();
      gradientWriter->SetInput(imageContainer[i]);
      char filename[100];
      if (DiffusionVectors->ElementAt(i).two_norm() <=
          0.0) // this is a reference image
      {
        snprintf(filename,
                 sizeof(filename),
                 "ReferenceImage%d.mhd",
                 referenceImageIndex);
        ++referenceImageIndex;
      }
      else
      {
        snprintf(filename, sizeof(filename), "Gradient%d.mhd", i);
      }
      gradientWriter->SetFileName(filename);
      gradientWriter->Update();
    }
  }
 
 
  // -------------------------------------------------------------------------
  auto tensorReconstructionFilter =
    TensorReconstructionImageFilterType::New();
 
  // The reference and the gradient images are conveniently provided as
  // input to the DiffusionTensor3DReconstructionImageFilter as
  //   filter->SetGradientImage( directionsContainer, nrrdreader->GetOutput()
  //   );
  //
  // The output of the nrrdreader is a VectorImage (akin to a multicomponent
  // 3D image). The nth component image is treated as a reference image if its
  // corresponding gradient direction is (0,0,0). Any number of reference
  // images may be specified.
  //
  // An alternate way to provide the inputs, when you have the reference and
  // gradient images in separate itk::Image< type, 3 > is  :
  //
  //   tensorReconstructionFilter->SetReferenceImage( image0 );
  //   tensorReconstructionFilter->AddGradientImage( direction1, image1 );
  //   tensorReconstructionFilter->AddGradientImage( direction2, image2 );
  //
  tensorReconstructionFilter->SetGradientImage(DiffusionVectors,
                                               reader->GetOutput());
 
  // This is necessary until we fix netlib/dsvdc.c
  tensorReconstructionFilter->SetNumberOfWorkUnits(1);
 
  tensorReconstructionFilter->SetBValue(b0);
  tensorReconstructionFilter->SetThreshold(
    static_cast<TensorReconstructionImageFilterType::ReferencePixelType>(
      std::stod(argv[2])));
  tensorReconstructionFilter->Update();
 
 
  // -------------------------------------------------------------------------
  // Write out the image of tensors. This code snippet goes to show that you
  // can use itk::ImageFileWriter to write an image of tensors.
  //
  using TensorWriterType = itk::ImageFileWriter<
    TensorReconstructionImageFilterType::OutputImageType>;
  auto tensorWriter = TensorWriterType::New();
  tensorWriter->SetFileName(argv[3]);
  tensorWriter->SetInput(tensorReconstructionFilter->GetOutput());
  tensorWriter->Update();
 
 
  // -------------------------------------------------------------------------
  // Now that we have the image of tensors, we may use one of the many tensor
  // filters in ITK. Below, we use the TensorFractionalAnisotropyImageFilter
  // to compute the FA.
  //
  using TensorPixelType =
    TensorReconstructionImageFilterType::TensorPixelType;
  using RealValueType = TensorPixelType::RealValueType;
  using FAImageType = itk::Image<RealValueType, Dimension>;
  using FAFilterType = itk::TensorFractionalAnisotropyImageFilter<
    TensorReconstructionImageFilterType::OutputImageType,
    FAImageType>;
 
  auto fractionalAnisotropyFilter = FAFilterType::New();
  fractionalAnisotropyFilter->SetInput(
    tensorReconstructionFilter->GetOutput());
 
  // Write the FA image
  //
  using FAWriterType = itk::ImageFileWriter<FAFilterType::OutputImageType>;
  auto faWriter = FAWriterType::New();
  faWriter->SetInput(fractionalAnisotropyFilter->GetOutput());
  faWriter->SetFileName(argv[4]);
  faWriter->Update();
 
  // Compute and write the Relative Anisotropy
  //
  using TensorPixelType =
    TensorReconstructionImageFilterType::TensorPixelType;
  using RealValueType = TensorPixelType::RealValueType;
  using RAImageType = itk::Image<RealValueType, Dimension>;
  using RAFilterType = itk::TensorRelativeAnisotropyImageFilter<
    TensorReconstructionImageFilterType::OutputImageType,
    RAImageType>;
 
  auto relativeAnisotropyFilter = RAFilterType::New();
  relativeAnisotropyFilter->SetInput(tensorReconstructionFilter->GetOutput());
 
  using RAWriterType = itk::ImageFileWriter<RAFilterType::OutputImageType>;
  auto raWriter = RAWriterType::New();
  raWriter->SetInput(relativeAnisotropyFilter->GetOutput());
  raWriter->SetFileName(argv[5]);
  raWriter->Update();
 
  return EXIT_SUCCESS;
}